ISOLATION & MOLECULAR IDENTIFICATION OF STAPHYLOCOCCUS EPIDERMIDIS S13 (PP654467) 16S RRNA GENE FROM POULTRY FECES SAMPLES OF LATUR REGION POULTRY FARM (MS) INDIA

Abstract

The accumulation of poultry waste, comprising various materials such as broiler and layer carcasses, feathers, bones, blood, hatchery debris, and deceased birds, poses significant environmental and health concerns. These waste materials can lead to microbial contamination, foul odours, and the proliferation of pests like flies and rodents, thereby contributing to environmental pollution. The Samples were collected according to standard microbiological procedures during study period September 2022 to June 2023. 2-5 gm of samples were collected in sterile screw cap tube using sterile spatula and immediately transported with specially prepared ice box to the laboratory for further analysis. Biochemical assays confirmed the bacterial specimens, and molecular characterization was conducted through polymerase chain reaction (PCR) and sequencing of the 16S rRNA gene of S. epidermidis. The newly sequenced 16S rRNA gene sequences demonstrated 100% homology to S. epidermidis, as analyzed using the NCBI-BLAST tool. Phylogenetic analysis and nucleotide base composition studies were performed using 60 sequences of the 16S rRNA gene from various Staphylococcus isolates, including Staphylococcus epidermidis. For this purpose, 16S rRNA gene sequences were retrieved from NCBI in FASTA format. The phylogenetic analysis, conducted using the Maximum Likelihood method, revealed the relationships and percent similarity of the  Staphylococcus epidermidis S13  (PP654467) 16S rRNA gene.